Catalog # A1001-100  –  42€  (100mL)

In cell and tissue cultures, Antibiotic-Antimycotic is used to prevent the growth of Gram-positive and Gram-negative bacteria, as well as yeasts and fungi. This solution provides a broad spectrum of efficacy because it contains a combination of three different agents: penicillin, streptomycin, and amphotericin B. Penicillin works by inhibiting the synthesis of bacterial cell walls. Streptomycin prevents prokaryote protein synthesis by disrupting the transition from the initiation complex to the chain-elongating ribosome, which also causes miscoding. Amphotericin B interferes with fungal membranes. It creates channels in the membranes, which causes small molecules to leak out.

Storage conditions: Frozen / Freeze again after using at –20°C
Shelf life: 24 months
Composition: 250 mg/l amphotericin B
205 mg/l sodium deoxycholate in water
Colour: Yellow
pH: 10 – 11.5
Osmolality: 25 mOsm/kg ± 25 mOsm/kg
Endotoxin: <10 EU/ml
Sterility tests:
   – bacteria aerobic-anaerobic
   – bacteria strictly anaerobic
   – fungi

Other tests:
Toxicity: Toxic effects will appear at 30 µg/ml for mammalian cells

Uses:
Use at 2.5 µg/ml

Remarks:
Amphotericin B is insoluble at pH 6 to 7, it is soluble at pH 2 or 11 for a concentration lower than 0.1 mg/ml in water.  It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use.

Shipping: Gel pack, dry ice or refrigerated transport  

Volume discounts available.

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