Catalog # R1533-500 –  28€  (500mL)

Hanks’ Balanced Salt Solution (HBSS) is designed to support the maintenance of mammalian cells by providing a chemically defined, balanced salt environment that preserves cellular structure and physiological function in vitro. Formulated with Hanks’ salts, it is suitable for use under ambient (non-CO2) atmospheric conditions. HBSS without calcium and magnesium is commonly used for washing and resuspending cells during enzymatic dissociation procedures, as these ions can interfere with enzymes such as trypsin. This 10X-concentrated formulation is free of calcium, magnesium, sodium bicarbonate, and phenol red.

Storage conditions: Room temperature
Shelf life: 48 months
Composition: Click here for the formulation
Colour: colourless, clear solution
pH: 5.9 ± 0.5
Osmolality: >1600 mOsm/kg
Endotoxin: <1 EU/ml
Sterility tests:
– Bacteria in aerobic and anaerobic conditions
– Fungi and yeasts

The product is intended to be used in vitro for research or further manufacturing only and not for use as an Active Pharmaceutical Ingredient or food or animal feed.

Uses:
Use this medium diluted at 1:10 with cell culture grade water.  After dilution and before use, add 4.7 ml of a sodium bicarbonate solution at 7.5% per litre of solution.
Supplements, such as antibiotics or sodium bicarbonate should be added as sterile supplements to the medium. Storage conditions and shelf-life of supplemented product will be affected by the nature of the supplements.

Signs of Deterioration:
Buffer solution should be clear and free of particulate and flocculent material. Do not use if buffer solution is cloudy or contains precipitate.  Other evidence of deterioration may include degradation of physical or performance characteristics.

Volume discounts available.

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