Catalog # R1502-100 – 10€  (100mL)
Catalog # R1502-500 – 15€  (500mL)
Catalog # R1502-10L – 122€  (10L)

Dulbecco’s Phosphate Buffered Saline (DPBS) is formulated to support the maintenance of mammalian cell physiology and morphology. It is widely used for rinsing cells, serving as a diluent in cell enumeration, and as a buffer in various chromatographic procedures. DPBS is also commonly used to wash cells during the dissociation process. This particular formulation is supplied as a 10X concentrate and is free of calcium and magnesium ions, preventing inhibition of enzymatic activity such as trypsin during cell dissociation.

Storage conditions: Room temperature
Shelf life: 48 months
Composition: Click here for the formulation
Colour: colourless, clear solution
pH: 6.8 ± 0.3
Osmolality: >1600 mOsm/kg
Endotoxin: < 1 EU/ml
Sterility tests:
– Bacteria in aerobic and anaerobic conditions
– Fungi and yeasts

Uses:
Supplements, such as antibiotics, should be added as sterile supplements to the buffer solution.  Storage conditions and shelf-life of the supplemented product will be affected by the nature of the supplements.

1. Dilute the 10X buffer 1:10 with cell culture grade water. Water temperature should be 15 to 30°C. Do not heat water. Mix completely.

2. While stirring the solution, adjust the pH 6.9 –7.1 using 1N HCl or 1N NaOH. For most cells and culture conditions, the optimal pH of this salt solution after filtration is 7.0-7.4.

3. Add deionized or distilled water to the solution to bring it to the final volume. To avoid fluctuations in pH, keep the vessel closed until the solution is filtered.

4. Sterilize the solution using a 0.22 micron filter. A peristaltic pump or an inert gas such as nitrogen can be used to provide positive pressure at 3 – 15 psi. Do not use CO2 gas.

5. Sterile solutions should be dispensed aseptically into sterile containers. Store buffer solution at 15 to 30°C.

Signs of Deterioration:
Buffer solution should be clear and free of particulate and flocculent material.  Do not use if buffer solution is cloudy or contains precipitate.  Other evidence of deterioration may include degradation of physical or performance characteristics.

The product is intended to be used in vitro for research or further manufacturing only and notfor use as an Active Pharmaceutical Ingredient or food or animal feed.

Volume discounts available.

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