Catalog # R1501-500  15€  (500mL)

Dulbecco’s Phosphate Buffered Saline (DPBS) 10X with Calcium and Magnesium is a concentrated, sterile solution essential for a wide range of cell culture applications. This formulation, containing both calcium and magnesium, is ideal for maintaining cell viability and physiological conditions during washing, dilution, and short-term cell handling procedures. Its 10X concentration allows for convenient dilution to 1X working solutions.

Shelf life: 48 months
Composition: Displayed on website; also available on request
Colour: colourless, clear solution
pH: 3.3 ± 0.4
Osmolality: > 1600 mOsm/kg
Endotoxin: < 1 EU/ml
Sterility tests:
   – Bacteria in aerobic and anaerobic conditions
   – Fungi and yeasts

Uses:
Supplements, such as antibiotics, should be added as sterile supplements to the buffer solution.  Storage conditions and shelf-life of the supplemented product will be affected by the nature of the supplements.

1. Dilute the 10X buffer 1:10 with cell culture grade water. Water temperature should be 15 to 30°C. Do not heat water. Mix completely.

2. While stirring the solution, adjust the pH 6.9 –7.1 using 1N NaOH. For most cells and culture conditions, the optimal pH of this salt solution after filtration is 7.0-7.4.

3. Add deionized or distilled water to the solution to bring it to the final volume. To avoid fluctuations in pH, keep the vessel closed until the solution is filtered.

4. Sterilize the solution using a 0.22 micron filter. A peristaltic pump or an inert gas such as nitrogen can be used to provide positive pressure at 3 – 15 psi. Do not use CO2 gas.

5. Sterile solutions should be dispensed aseptically into sterile containers. Store buffer solution at 15 to 30°C

Signs of Deterioration:
Buffer solution should be clear and free of particulate and flocculent material.  Do not use if buffer solution is cloudy or contains precipitate.
Other evidence of deterioration may include degradation of physical or performance characteristics.

The product is intended to be used in vitro for research or further manufacturing only and not for use as an Active Pharmaceutical Ingredient or food or animal feed.

Volume discounts available.

To place an order or learn more about our products, please call us at +351 308 800 210, contact your local salesperson, or write us an email at sales@phoenix-scientific.com, or use our contact us page.