Catalog # A1002-100  39€  (100mL)

In cell and tissue cultures, Antibiotic-Antimycotic is used to prevent the growth of Gram-positive and Gram-negative bacteria, as well as yeasts and fungi. This solution provides a broad spectrum of efficacy because it contains a combination of three different agents: penicillin, streptomycin, and amphotericin B. Penicillin works by inhibiting the synthesis of bacterial cell walls. Streptomycin prevents prokaryote protein synthesis by disrupting the transition from the initiation complex to the chain-elongating ribosome, which also causes miscoding. Amphotericin B interferes with fungal membranes. It creates channels in the membranes, which causes small molecules to leak out.

Shelf life: 24 months

Composition: Click here for formulation sheet

Colour: Yellow
pH: 8.5 ± 1
Osmolality: 300 – 450 mOsm/kg

Endotoxin: <10 EU/ml

Sterility tests:
– bacteria aerobic-anaerobic
– bacteria strictly anaerobic
– fungi

Cell Growth test: Not applicable
Other tests:
Toxicity: Toxic effects will appear at 30 µg/ml for mammalian cells
Recommended use: Dilute with adequate buffer or cell culture medium 1:100
– Respect storage conditions of the product
– Do not use the product after its expiry date
– Store product in an area protected from light
– Manipulate the product in aseptic conditions (e.g. : under laminar air flow)
– Wear clothes adapted to the manipulation of the product to avoid contamination (e.g. : gloves, mask,
hygiene cap, overall…)
– In order to preserve all product qualities, it is recommended to thaw out the flask, to aliquote, then to re-freeze the produced flasks rather than to thaw out and re-freeze the flask at each use.
– It is recommended to use the product immediately after its thaw out.

The product is intended to be used in vitro for research or further manufacturing only and not for use
as an Active Pharmaceutical Ingredient or food or animal feed.

Application:
For tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi.
Penicillin acts by inhibiting bacterial cell wall synthesis.
Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding.

Uses:
Potency of the penicillin in solution: 10 000 000 U/l
Use at 10ml/l

Gel pack, dry ice or refrigerated transport -18°C to -40°C. Volume discounts available.

To place an order or learn more about our products, please call us at +351 308 800 210, contact your local salesperson, or write us an email at sales@phoenix-scientific.com, or use our contact us page.